Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum

Authors

  • Amal Jabbar Ghaib Department of microbiology in the ministry of health/ Baghdad, Iraq
  • Amani Mohammed Jasim College of Health & Medical Technology - Baghdad, Middle Technical University, Baghdad, Iraq
  • Amal Aziz Kareem College of Health & Medical Technology - Baghdad, Middle Technical University, Baghdad, Iraq

DOI:

https://doi.org/10.51173/jt.v4i3.551

Keywords:

Cryptosporidium, Lactobacillus ruteri, Probiotic bacteria, MTT test

Abstract

The goal of this study was to see how well Lactobacillus ruteri probiotic bacteria might infect Cryptosporidium parvum viability at various bacterial dilutions and statuses. Cryptosporidium was obtained from stool samples of diarrheal patients and diagnosed using parasitological methods, followed by molecular methods and cell line cultivation (Human adenocarcinoma cell). The treatment of Cryptosporidium Parvum by adding Lactobacillus ruteri dilutions and status were then incubated at incubation periods (24, 48, and 72 hr), detection of Cryptosporidium count was carried out microscopically, and the parasite's viability was determined by 3-(4,5 dimethylthiazol-2-yl)-2- Diphenyltetrazolium bromide(Mtt) technique. Lactobacillus ruteri can treat Cryptosporidium parvum by suppressing activity and lowering the count with extremely significant differences, according to the findings. 

Downloads

Download data is not yet available.

References

Yang X, Guo YQ, Xiao LH, Feng YY. Molecular epidemiology of human cryptosporidiosis in low- and middle-income countries. Clin Microbiol Rev 2021;34(2):e00087 − 19. http://dx.doi.org/10.1128/ CMR.00087-19.

Adam RD. Giardia duodenalis: biology and pathogenesis. Clin Microbiol Rev 2021;34(4):e00024 − 19. http://dx.doi.org/10.1128/ cmr.00024-19.

Maria Priscila Saracino1, Cecilia Celeste Vila, Pablo César Baldi1,2, et al., Searching for the one(s):2021 Using Probiotics as Anthelmintic Treatments. Front. Pharmacol.2021 https://doi.org/10.3389/fphar.71419.

Ryan U, Zahedi A, Feng Y, Xiao L. An Update on Zoonotic Cryptosporidium Species and Genotypes in Humans. Animals. 2021;11(11):3307.

Adinew Ebiyo1 and Geremew Haile. Prevalence and Factors Associated with Cryptosporidium Infection in Calves in and around Nekemte Town, East Wollega Zone of Ethiopia. Research Article 2022 | Open Access,Volume |Article ID 1468242 | https://doi.org/10.1155/2022/1468242

Mu Q, Tavella VJ, Luo XM. Role of Lactobacillus reuteri in Human Health and Diseases. Front Microbiol. 2018 Apr 19;9:757. doi: 10.3389/fmicb.2018.00757. PMID: 29725324; PMCID: PMC5917019.

Bairami, A., Rezaei, S., Rezaeian, M., Synchronous Identification Of Entamoeba Histolytica, Giardia Intestinalis, And Cryptosporidium Spp. In Stool, Samples Using A Multiplex PCR Assay. Iran. Journal. Parasitol.2018 13, p24.

ChristopherN.MillerLyneJosséIanBrown BenBlakeman, et al. A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biologyInternational Journal for Parasitology2018Volume 48, Issues 3–4, pp 197-201.

Ahmed Sayed Bayoumy; Tarek Zaalouk; Ahmed Agamawy. In vitro Model for Cryptosporidium parvum Infection.2020, Volume 1, Issue 1, Page 12-15

Yeshambel Taye, Isolation and Identification of Lactic Acid Bacteria from Cow Milk and Milk Products Research Article | Open Access, 2021 Volume 2021 |Article ID 4697445 | https://doi.org /10.1155/ 2021/4697445.

Yellanthoor RB. Acute gastroenteritis due to Blastocystis hominis in an adolescent boy. BMJ Case Reports CP. 2020;13(12):e237810.

Jennifer K.O'LearyRoy D.SleatorBrigidLucey, Cryptosporidium spp. Diagnosis and Research in the 21st Century Food and Waterborne Parasitology,2021, Volume 24, e00131

Zi Jin1, Jingbo Ma1,2, Guan Zhu1* and Haili Zhan. Discovery of Novel Anti-cryptosporidial Activities From Natural Products by in vitro High-Throughput Phenotypic Screening. Front. Microbiol.2019, 29 | https://doi.org/10.3389/fmicb.2019.01999

Feng, Xiao MM; Zhuang, LiJuan MM; Chen, Ling MM; Zhao, Hongying MM; Huang, Rui MM; Guo, ZhiFeng MM* Comparison of different probiotics in the treatment of acute diarrhea in children, Medicine, 2022 - Volume 101 - Issue 11 - e28899

Ye, P., Wang, J., Liu, M., Li, P. & Gu, Q. Purification and characterization of a novel bacteriocin from Lactobacillus paracasei ZFM54. LWT-Food Sci. Technol. 143, 111125 (2021).

Jeffrey M Pernica,1,2 Tonya Arscott-Mills,3,4,5 Andrew P Steenhoff,5. Optimising the management of childhood acute diarrhoeal disease using a rapid test-and-treat strategy and/or Lactobacillus reuteri DSM 17938: a multicentre, randomised, controlled, factorial trial in Botswana Pernica JM, et al. BMJ Global Health 2022;7:e007826. doi:10.1136/bmjgh-2021-007826

James C.Foster a, Matthew D. Effect of Lactobacillus and Bifidobacterium on Cryptosporidium parvum oocyst viability, Food Microbiology 20 (2003) pp351–357.

Qi C, Ding M, Li S, Zhou Q, Li D, Yu R, et al. Sex-dependent modulation of immune development in mice by secretory IgA-coated Lactobacillus reuteri isolated from breast milk. J Dairy Sci. (2021) 104:3863–75. doi: 10.3168/jds.2020-19437

Mathipa MG, antsha MS (2017). Probiotic engineering: towards development of robust probiotic strains with enhanced functional properties and for targeted control of enteric pathogens.Gut. Pathog. (2017).

Azza Fahmy , Gehan Abuelenain, Amr Abdou , Mohamed Elhakeem. Comparative Study between Wild and Commercial Egyptian Lactobacillus reuteri-Antagonizing Effects in Cryptosporidiosis Models. Research Article. May 2021 | Volume 9 | Issue 6 | P 802.

Al-Alousi TI, Abdullah H . Prophylactic role of lactic acid prepared from lactobacillus acidophilus on cryptosporidiosis. Tikrit J. Pure Sci. 2018.23(9): 1813–1662. https://doi.org/ 10.25130/tjps.23.2018.141).

Terrapon N, Henrissat B. How do gut microbes break down dietary ber? Trend Biochem.2014 Sci. 39(4): 156–158.

Del Coco VF (2015) Microorganisms conferring beneficial health effects.Rev Argent Mi.

Downloads

Published

2022-09-30

How to Cite

Amal Jabbar Ghaib, Amani Mohammed Jasim, & Amal Aziz Kareem. (2022). Studying the Effect of Human–Lactobacillus Ruteri on the Viability of Cryptosporidium Parvum. Journal of Techniques, 4(3), 47–52. https://doi.org/10.51173/jt.v4i3.551

Issue

Section

Medical techniques